Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10
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چکیده
منابع مشابه
Characterization of the low pH/low nutrient-resistant LNCaP cell subline LNCaP-F10.
Intratumoral regions of low extracellular pH and low nutrition are common features of solid tumors. Although cancer cells normally die when they are removed from their environment, a small population of cells survive. In the present study, the subline LNCaP-F10, of the prostate cancer cell line LNCaP, was isolated and its low pH/low nutrient-resis...
متن کاملGrowth and characterization of LNCaP prostate cancer cell spheroids.
Cells from the prostate tumor cell line LNCaP have been grown as spheroids. The growth kinetics of the spheroids have been characterized by fitting a Gompertz equation to spheroid growth curves. The proliferation state of cells within spheroids of different diameters was assessed by bromodeoxyuridine staining. Scanning and electron transmission microscopy were performed to determine the ultrast...
متن کاملExpression in a Human Prostatic Cancer (LNCaP) Subline Autocrine Regulation of Prostate-specific Antigen Gene
Prostate-specific antigen (PSA), a Mr 34,000 serine protease, is recog nized as a useful marker for the detection and prognosis of patients with prostate cancer. Although serum PSA is an excellent prognostic indicator, an increasing number of factors were found to regulate the PSA expression of prostatic cancer cells, which include androgenic steroids, the growth factors (GFs) and the extracell...
متن کاملAutocrine regulation of prostate-specific antigen gene expression in a human prostatic cancer (LNCaP) subline.
Prostate-specific antigen (PSA), a M(r) 34,000 serine protease, is recognized as a useful marker for the detection and prognosis of patients with prostate cancer. Although serum PSA is an excellent prognostic indicator, an increasing number of factors were found to regulate the PSA expression of prostatic cancer cells, which include androgenic steroids, the growth factors (GFs) and the extracel...
متن کاملLineage relationship between LNCaP and LNCaP-derived prostate cancer cell lines.
BACKGROUND LNCaP and its derivative cell lines, which include C4-2 (and the related C4-2B) and CL1, are used as models of prostate cancer. Unlike LNCaP, the other cell lines show features of progressed disease such as metastatic capability and hormone independence. Analyses were done to determine if C4-2 or CL1 cells were selected from pre-existent subpopulations in LNCaP. METHODS Prostate ca...
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ژورنال
عنوان ژورنال: Oncology Reports
سال: 2012
ISSN: 1021-335X,1791-2431
DOI: 10.3892/or.2012.2039